SU5408 is primarily used in biochemical research as a potent inhibitor of fibroblast growth factor receptor (FGFR) and vascular endothelial growth factor receptor (VEGFR) tyrosine kinases. It is widely employed in studies to investigate the role of these receptors in cell signaling, angiogenesis, and tumor growth. Researchers utilize SU5408 to block FGFR and VEGFR activity, helping to understand their contributions to cancer progression and other diseases involving abnormal cell proliferation. Additionally, it is used in developmental biology to study the impact of FGFR signaling on embryonic development and tissue regeneration. Its application extends to in vitro and in vivo experiments, providing insights into potential therapeutic targets for cancer and other conditions.