5-IAF is widely used as a fluorescent labeling reagent in biochemical and cellular research. It is particularly effective for tagging proteins, peptides, and other biomolecules due to its ability to react with thiol groups, such as those found in cysteine residues. This labeling allows for the visualization and tracking of these molecules in various assays, including fluorescence microscopy, flow cytometry, and gel electrophoresis. Additionally, 5-IAF is utilized in studying protein interactions, conformational changes, and localization within cells. Its bright fluorescence and stability make it a valuable tool in both in vitro and in vivo studies, aiding in the understanding of complex biological processes.