Used primarily as a fluorescent dye in various biological and chemical applications. It is commonly employed in microscopy and flow cytometry to stain cells and tissues, enabling visualization under fluorescence microscopy. The dye is also utilized in molecular biology for labeling nucleic acids and proteins, aiding in the study of their structure and function. Additionally, it serves as a pH indicator in certain analytical procedures, changing color in response to pH variations. Its bright fluorescence makes it valuable in diagnostic assays and research studies involving cellular processes.