Rose Bengal is widely used in ophthalmology as a diagnostic stain to identify damaged cells on the surface of the eye, particularly in the detection of corneal abrasions and dry eye syndrome. It helps eye care professionals assess the health of the ocular surface by highlighting areas of epithelial cell loss or dysfunction. In microbiology, Rose Bengal serves as a selective agent in culture media to inhibit the growth of bacteria while promoting the growth of fungi, making it valuable for isolating and identifying fungal species in environmental or clinical samples. Additionally, it is employed in photodynamic therapy for certain skin conditions and cancers. When activated by light, Rose Bengal generates reactive oxygen species that can destroy targeted cells, offering a potential treatment option for localized malignancies or infections. In research, Rose Bengal is used as a fluorescent dye in various biological and biochemical assays to visualize cellular structures or track molecular interactions due to its ability to bind to proteins and other biomolecules. Its applications also extend to the food industry, where it is used as a color additive in certain products, though this use is regulated and varies by region.