Bis-Mal-PEG6 is widely used in bioconjugation and drug delivery systems due to its ability to link molecules through maleimide groups, which react specifically with thiols. This property makes it valuable for creating stable conjugates with proteins, peptides, or antibodies, enhancing their therapeutic potential. It is also utilized in the development of antibody-drug conjugates (ADCs) for targeted cancer therapy, where it serves as a linker to attach cytotoxic drugs to antibodies. Additionally, Bis-Mal-PEG6 is employed in surface modification of nanoparticles, improving their biocompatibility and targeting efficiency. Its PEG spacer enhances solubility and reduces immunogenicity, making it a crucial component in advanced biomedical applications.