Used primarily in biochemical research and pharmaceutical development to study and modify heparin and heparan sulfate structures. It helps in analyzing the composition and sequence of these complex polysaccharides, which is crucial for understanding their biological roles and interactions, including processes such as inflammation, cell growth, and cancer metastasis.

Additionally, it is employed in the production of low molecular weight heparins, which are used as anticoagulant drugs, and in manufacturing processes for drugs and vaccines to remove contaminating heparin.

In clinical settings, it aids in the development of assays to measure heparin levels in blood, ensuring safe and effective dosing for patients undergoing anticoagulation therapy. It is also used to neutralize excess heparin in blood, such as in cases of overdose, to mitigate its effects.