Hyaluronidase is widely used in medical and cosmetic procedures due to its ability to break down hyaluronic acid. In medicine, it is often employed to enhance the absorption and dispersion of injected drugs, such as local anesthetics or fluids, by reducing tissue resistance. This makes it particularly useful in ophthalmic surgeries, where it helps in the administration of anesthesia and the management of vitreous hemorrhage. Additionally, it is utilized in subcutaneous fluid administration (hypodermoclysis) for patients who have difficulty with intravenous hydration.

In cosmetic treatments, hyaluronidase is used as an antidote to dissolve hyaluronic acid-based dermal fillers in cases of overcorrection, asymmetry, or complications like vascular occlusion. This application allows for quick correction and minimizes adverse effects. It is also used in fertility treatments to improve the penetration of sperm during intracytoplasmic sperm injection (ICSI). Overall, hyaluronidase plays a versatile role in enhancing the efficacy and safety of various therapeutic and aesthetic procedures.