Used in molecular biology research to modulate protein degradation via the ubiquitin-proteasome system, specifically as a selective inhibitor of the VHL (von Hippel-Lindau) protein. VHL plays a key role in the ubiquitination and degradation of HIF-1α. This compound stabilizes HIF-1α levels in cells, mimicking hypoxia-induced responses at the cellular level. It is widely applied in studying hypoxia-related disease models, including cardiovascular diseases, stroke, and cancer, as well as in drug development targeting angiogenesis regulation.