Used primarily as a vital stain in biological research to distinguish between live and dead cells. It helps in assessing cell viability by selectively staining dead cells blue, while live cells remain unstained. Commonly applied in cell culture experiments, tissue engineering, and microbiology to evaluate cell membrane integrity. Also utilized in ophthalmology during cataract surgery to stain the anterior capsule of the lens, enhancing visibility for surgical procedures. Additionally, it aids in studying cell permeability and identifying damaged tissues in various diagnostic applications.