Monofucosyllacto-N-hexaose III is primarily utilized in the field of nutritional science and infant formula development. It serves as a key component in mimicking the complex oligosaccharide structures found in human breast milk, which are crucial for supporting infant gut health and immune system development. This compound is incorporated into advanced infant formulas to promote the growth of beneficial gut bacteria, enhance intestinal barrier function, and reduce the risk of infections. Additionally, it is studied for its potential role in modulating immune responses and its applications in dietary supplements aimed at improving overall gut microbiota balance in both infants and adults.