Used in biomedical research to quantify levels of phosphoenolpyruvate carboxykinase (PEPCK) in human biological samples such as serum, plasma, or cell lysates. This assay is commonly applied in metabolic studies, particularly those investigating gluconeogenesis, insulin resistance, diabetes, and liver function. Researchers use the kit to monitor changes in PEPCK expression under various physiological or pathological conditions, aiding in the understanding of metabolic regulation and potential therapeutic interventions. The ELISA format allows for high sensitivity and specificity, making it suitable for both clinical research and laboratory investigations.