Alizarin Red S Solution is widely used as a staining agent in biological and histological applications. It selectively binds to calcium deposits in tissues, making it valuable for visualizing calcified areas in bone sections, cell cultures, and pathological samples. It is commonly employed in assays to detect mineralization in osteoblast cultures, aiding in bone formation studies. In analytical chemistry, Alizarin Red S serves as a colorimetric reagent for detecting metal ions such as aluminum, calcium, and fluoride in solution. Its distinct color change upon complexation allows for qualitative and quantitative analysis. The solution is also used in educational labs to demonstrate chelation and pH-dependent color shifts. Additionally, it finds use in textile and material sciences as a dye for natural fibers and in the development of sensors due to its responsive chromogenic properties.