Methyl Red is widely used as a pH indicator in various laboratory applications, particularly in microbiology and chemistry. In microbiology, it is a key component of the Methyl Red test, which helps identify bacteria that produce stable acids through mixed acid fermentation of glucose. This test is crucial in differentiating between Escherichia coli and Enterobacter aerogenes. In chemistry, it serves as a visual pH indicator in titrations, changing color from red at pH 4.4 and below to yellow at pH 6.2 and above, making it useful for determining the endpoint in acid-base reactions. Additionally, it is employed in environmental monitoring to assess the pH of water bodies and soil samples, aiding in pollution control and ecological studies. Its stability and distinct color transition make it a reliable tool in both research and industrial settings.