Bromocresol green is widely used as a pH indicator in various analytical and laboratory applications. It is particularly useful in titration experiments to determine the endpoint of acid-base reactions, as it changes color within a pH range of 3.8 to 5.4, transitioning from yellow in acidic conditions to blue in alkaline conditions.

In microbiology, it is employed in culture media to monitor pH changes, ensuring optimal growth conditions for microorganisms. Additionally, it is utilized in biochemistry for protein electrophoresis to visualize pH gradients and assess the progress of separation processes. Its ability to provide clear visual cues makes it a valuable tool in both qualitative and quantitative analyses.