Alizarin Red S is widely used as a biological stain to detect calcium deposits in tissues, making it a valuable tool in histology and pathology for studying bone formation and mineralization processes. It is also employed in analytical chemistry as an indicator for complexometric titrations, particularly for determining metal ions like aluminum and calcium. In the textile industry, it serves as a dye for coloring fabrics, especially wool and silk, due to its vibrant red hue. Additionally, it is utilized in environmental science to assess soil and water quality by detecting the presence of certain metal ions. Its versatility in staining, dyeing, and analytical applications makes it a significant compound in various scientific and industrial fields.