AMPHOLINE is widely used in biochemistry and molecular biology for isoelectric focusing (IEF), a technique that separates proteins based on their isoelectric points. It serves as a carrier ampholyte, creating a pH gradient in polyacrylamide gels, enabling precise separation and analysis of proteins. This application is crucial in proteomics for studying protein composition, post-translational modifications, and biomarker discovery. Additionally, AMPHOLINE is utilized in the purification of proteins and peptides, aiding in the isolation of specific molecules for further research or therapeutic development. Its role in IEF also supports the characterization of complex biological samples, contributing to advancements in medical diagnostics and drug development.