Isobutyl isothiocyanate is primarily used in the flavor and fragrance industry due to its pungent and mustard-like aroma. It is often incorporated into food products to enhance or replicate the taste of mustard, horseradish, and similar condiments. Additionally, it serves as a key ingredient in the formulation of certain spice blends and sauces, providing a sharp, spicy note. In the field of agriculture, it is explored as a potential biofumigant for pest control, leveraging its natural pesticidal properties to protect crops from insects and pathogens. Its use in research also extends to studying its antimicrobial and anticancer properties, making it a subject of interest in pharmaceutical and biomedical studies.