Acetate Kinase from Escherichia coli, in its lyophilized powder form, is widely utilized in biochemical research and industrial applications. It plays a critical role in enzymatic assays, particularly in the study of metabolic pathways involving acetate and ATP. This enzyme is essential for catalyzing the transfer of a phosphate group from acetyl phosphate to ADP, generating ATP and acetate, making it valuable in energy metabolism studies. In biotechnology, it is employed in the production of acetyl-CoA, a key intermediate in various biosynthetic processes. Additionally, it is used in diagnostic kits to measure ATP levels, aiding in the detection of cellular energy status. Its stability in lyophilized form ensures long-term storage and consistent performance in laboratory settings. Furthermore, Acetate Kinase is applied in synthetic biology for designing and optimizing metabolic pathways in engineered microorganisms, enhancing the production of biofuels and other bioproducts. Its versatility and efficiency make it a crucial tool in advancing both fundamental research and industrial biotechnology.