22(S)-hydroxy Cholesterol is primarily used in research settings to study cholesterol metabolism and its role in various biological processes. It serves as a key intermediate in the biosynthesis of bile acids, making it valuable for investigating pathways related to lipid metabolism and liver function. Additionally, this compound is utilized in studies exploring the regulation of cholesterol homeostasis and its impact on cellular signaling. Researchers also employ it to examine the effects of oxysterols on immune responses and inflammation, as well as their potential role in neurodegenerative diseases. Its application extends to drug discovery, where it aids in identifying therapeutic targets for conditions linked to cholesterol dysregulation.