7β-hydroxy cholesterol is primarily studied for its role in biological systems, particularly in the context of cholesterol metabolism and inflammation. It is known to be an oxysterol, a class of oxidized derivatives of cholesterol, which play significant roles in cellular signaling and regulation. One of the key applications of 7β-hydroxy cholesterol is in research related to atherosclerosis and cardiovascular diseases. It is often used as a biomarker to study oxidative stress and lipid peroxidation, which are critical factors in the development of these conditions. Additionally, it has been investigated for its potential role in modulating immune responses and inflammation, making it relevant in studies of autoimmune diseases and chronic inflammatory conditions. In the field of cancer research, 7β-hydroxy cholesterol is explored for its effects on cell proliferation and apoptosis. Its interactions with nuclear receptors and signaling pathways provide insights into its potential impact on cancer cell behavior. Furthermore, it is used in biochemical assays to understand the mechanisms of cholesterol metabolism and its related disorders, contributing to the development of therapeutic strategies targeting these pathways.