Aflatoxin G2 is primarily known for its toxicological significance rather than industrial or commercial applications. It is one of the secondary metabolites produced by certain strains of Aspergillus fungi, commonly found in contaminated agricultural products such as corn, peanuts, and tree nuts. Its presence is a major concern in food safety due to its potent hepatotoxic and carcinogenic properties.

In research settings, Aflatoxin G2 is used as a reference standard in analytical testing to detect and quantify aflatoxin contamination in food and feed. It plays a critical role in validating methods like HPLC and ELISA for monitoring mycotoxin levels. Due to its high toxicity, it is also studied in toxicology to understand mechanisms of DNA damage, liver disease, and cancer development.

Regulatory agencies use data from Aflatoxin G2 studies to establish safety limits and control measures in the food supply chain. It has no approved use in pharmaceuticals, agriculture, or manufacturing due to its hazardous nature.