Deoxypseudouridine is primarily utilized in the field of nucleic acid research and therapeutic development. It serves as a modified nucleoside, often incorporated into oligonucleotides to enhance their stability and functionality. This modification is particularly valuable in the design of antisense oligonucleotides and small interfering RNAs (siRNAs), where it helps improve resistance to nucleases, thereby increasing the lifespan and efficacy of these molecules in biological systems. Additionally, deoxypseudouridine is explored in the development of novel therapeutic agents, including potential treatments for genetic disorders and viral infections, due to its ability to modulate RNA interactions and processing. Its unique properties make it a promising candidate for advancing gene therapy and RNA-based therapeutics.