N4-Benzoyl-2'-deoxycytidine is primarily used in the field of nucleic acid research and synthesis. It serves as a protected form of deoxycytidine, where the benzoyl group safeguards the amine functionality during oligonucleotide synthesis. This protection is crucial in preventing unwanted side reactions, ensuring the accurate and efficient assembly of DNA sequences. The compound is particularly valuable in solid-phase synthesis techniques, where it contributes to the production of high-purity oligonucleotides for applications such as gene editing, PCR, and molecular diagnostics. Its stability and reactivity make it a reliable building block in the development of custom DNA strands for research and therapeutic purposes.