This compound is primarily utilized in the synthesis of modified oligonucleotides, particularly in the field of nucleic acid research and therapeutic development. Its structure, featuring protective groups like the 4,4'-dimethoxytrityl (DMT) and benzoyl moieties, makes it a key intermediate in solid-phase oligonucleotide synthesis. The DMT group protects the 5'-hydroxyl during the stepwise assembly of nucleotides, while the benzoyl group safeguards the exocyclic amine of cytidine. The 2'-O-methyl modification enhances the stability of the resulting oligonucleotides against nuclease degradation, making it valuable for applications in antisense technology, RNA interference (RNAi), and the development of therapeutic oligonucleotides. Additionally, it is used in the study of RNA structure and function, as well as in the design of synthetic RNAs for gene silencing and regulation.