Cytidine 5'-monophosphate disodium salt is widely used in biochemical research as a nucleotide building block for synthesizing RNA and studying RNA metabolism. It plays a key role in enzymatic reactions and is often employed in the preparation of nucleic acid analogs. Additionally, it is utilized in cell culture media to support cell growth and proliferation, particularly in studies involving nucleotide metabolism. Its application extends to the development of pharmaceuticals, where it serves as an intermediate in the production of antiviral and anticancer drugs. In food science, it is used as a flavor enhancer, contributing to the umami taste in various products.