Widely used in biochemical research to study cellular energy transfer, this compound serves as a substrate in enzyme assays involving kinases, ATPases, and other ATP-dependent enzymes. It is essential in molecular biology for in vitro phosphorylation reactions and protein activation. Commonly applied in cell culture systems to investigate signaling pathways, particularly those related to purinergic receptors. Also utilized in studies of mitochondrial function and metabolic regulation. In some diagnostic kits, it acts as a reagent for bioluminescent assays, leveraging the luciferin-luciferase system for sensitive detection of ATP-dependent luminescence. Its tris salt form enhances solubility and stability in buffered solutions, making it suitable for physiological pH applications.