Cytidine 5′-triphosphate disodium salt is widely used in molecular biology and biochemistry research. It serves as a substrate in enzymatic reactions, particularly in the synthesis of RNA during in vitro transcription processes. This compound is essential for studying RNA polymerase activity and understanding RNA synthesis mechanisms. Additionally, it is utilized in the production of labeled RNA probes for hybridization experiments, aiding in gene expression analysis. In metabolic studies, it acts as a precursor for the biosynthesis of nucleotides and other critical cellular components. Its role in energy transfer and signaling pathways also makes it valuable in exploring cellular metabolism and enzyme kinetics.