Rutinose is a disaccharide composed of α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranose, primarily serving as a key intermediate in the enzymatic and chemical synthesis of various glycosides, including the flavonoid rutin (quercetin-3-rutinoside), which exhibits potent antioxidant properties. In the pharmaceutical sector, it is utilized in research to facilitate glycosylation of bioactive compounds, potentially enhancing their solubility, stability, and bioavailability. While direct incorporation into foods is limited, rutinose contributes to the development of functional ingredients and dietary supplements through its role in producing antioxidant-rich glycosides. It is also essential in scientific research for studying glycosylation mechanisms, glycosyltransferase enzymes, and carbohydrate metabolism pathways.