Rutinose is primarily used in the synthesis of various glycosides, particularly in the production of flavonoids like rutin, which have significant antioxidant properties. Its application extends to the pharmaceutical industry, where it is utilized to enhance the bioavailability and stability of certain drugs. In the food industry, rutinose is incorporated into functional foods and dietary supplements to promote health benefits associated with its antioxidant activity. Additionally, it plays a role in research and development for studying glycosylation processes and enzyme mechanisms.