Acetobromocellobiose is primarily used in biochemical research as a substrate for studying enzyme activity, particularly in the field of glycoscience. It serves as a key intermediate in the synthesis of complex carbohydrates and glycoconjugates, aiding in the exploration of glycosylation processes. Additionally, it is employed in the development of glycoprotein-based therapeutics and vaccines, where understanding carbohydrate-protein interactions is crucial. Its application extends to the study of cellulose degradation mechanisms, providing insights into biofuel production and enzymatic breakdown of plant biomass.