Sphingomyelinase from Bacillus cereus is widely used in biochemical research to study cell membrane dynamics and signaling pathways. It specifically hydrolyzes sphingomyelin to produce ceramide, which plays a critical role in cellular processes such as apoptosis, differentiation, and stress responses. This enzyme is also employed in lipidomics to analyze sphingolipid metabolism and its implications in diseases like cancer, neurodegenerative disorders, and metabolic syndromes. Additionally, it serves as a tool in biotechnological applications for modifying lipid bilayers in model membranes, aiding in the study of membrane structure and function. Its ability to induce ceramide generation makes it valuable for investigating therapeutic targets in various pathological conditions.