Used to prevent protein degradation during cell lysis and extraction by inhibiting proteolytic enzymes such as serine, cysteine, and aspartic proteases. Ideal for preserving native protein structure and function in applications like Western blotting, immunoprecipitation, ELISA, and protein purification. Added directly to lysis buffers at a 1X concentration, it ensures high yield and integrity of target proteins from mammalian samples. Effective in both adherent and suspension cell cultures, as well as tissue homogenates. Stable at -20°C and compatible with various downstream assays.