This compound is primarily used in biochemical research as a fluorogenic substrate for caspase-2, an enzyme involved in apoptosis (programmed cell death). It is designed to measure caspase-2 activity in vitro, helping researchers study the mechanisms of cell death and related pathways. When cleaved by caspase-2, it releases a fluorescent signal, allowing for the quantification of enzyme activity. This makes it a valuable tool in drug discovery and development, particularly in screening for compounds that modulate apoptosis. Additionally, it aids in understanding diseases where apoptosis is dysregulated, such as cancer and neurodegenerative disorders. Its specificity for caspase-2 ensures accurate and reliable results in experimental studies.