Neutral protease is widely used in various industries due to its ability to break down proteins under neutral pH conditions. In the food industry, it plays a key role in meat tenderization, improving texture and digestibility. It is also used in brewing to prevent haze formation by degrading protein-polyphenol complexes, thus enhancing beer clarity and shelf life.

In detergent manufacturing, neutral protease helps remove protein-based stains such as blood, egg, and grass from fabrics, making it a common ingredient in laundry and dishwashing detergents. Its effectiveness at moderate temperatures and neutral pH makes it suitable for eco-friendly cleaning products.

In the leather industry, it aids in dehairing and bating processes, replacing harsh chemicals and reducing environmental impact. Additionally, neutral protease is used in molecular biology and biotechnology for cell isolation and tissue dissociation, supporting research and diagnostic applications.

It also finds use in digestive health supplements, where it supports protein digestion and may help reduce gastrointestinal discomfort after meals. In cosmetics, it can help exfoliate by breaking down dead skin cells, promoting smoother skin.