Used in peptide synthesis as a protected form of tyrosine, enabling selective modification of peptides without affecting other functional groups. It is particularly valuable in studying protein interactions and enzyme mechanisms, where nitrotyrosine serves as a marker for oxidative stress. The Boc group provides stability during synthesis, ensuring the nitrotyrosine remains intact until deprotection is required. This compound is also utilized in research focused on understanding the role of tyrosine nitration in diseases like neurodegenerative disorders and cardiovascular conditions. Its application extends to the development of biochemical probes and inhibitors for studying nitric oxide pathways.