Ethyl 6-hydroxyquinoline-3-carboxylate is primarily used in the field of organic synthesis as a key intermediate for the preparation of various biologically active compounds. Its quinoline core structure makes it valuable in the development of pharmaceuticals, particularly in the synthesis of potential anticancer, antimicrobial, and anti-inflammatory agents. Additionally, it serves as a building block in the creation of fluorescent dyes and probes, which are utilized in biochemical and medical research for imaging and diagnostic purposes. Its hydroxyl and ester functional groups allow for further chemical modifications, enabling the production of diverse derivatives with tailored properties for specific applications.