Aflatoxin M1 is a certified reference material used in analytical laboratories for the accurate detection and quantification of this mycotoxin in milk and dairy products. As a hydroxylated metabolite of Aflatoxin B1, it forms when contaminated animal feed is ingested by dairy animals, resulting in its excretion into milk. This reagent is essential for calibration and validation of testing methods, such as HPLC or ELISA, to ensure compliance with strict regulatory limits set by bodies like the FDA and EU, given its carcinogenic potential. It supports quality control in milk production, processing, and distribution, and is widely employed in food safety research and toxicology studies to develop strategies for mitigating contamination and protecting public health.