Rapid Endonazyme AgeI is a high-efficiency restriction enzyme used in molecular biology for precise DNA cleavage at specific recognition sites. It is commonly applied in DNA cloning, where it enables the generation of compatible ends for ligation into vectors. Due to its rapid digestion kinetics, it supports shorter reaction times, making it ideal for high-throughput applications such as plasmid screening and restriction mapping. The enzyme is also used in gene construction and genome engineering workflows, including the preparation of DNA fragments for synthetic biology. Its robust activity under standardized buffer conditions allows for reliable performance in double and multiple digestions with other restriction enzymes.