Endonuclease NruI is widely used in molecular biology for DNA manipulation and analysis. It is particularly valuable in genetic engineering for creating specific DNA fragments by cleaving at precise recognition sites. Researchers use it to prepare DNA for cloning, sequencing, and other downstream applications. Its ability to produce defined cuts in DNA makes it essential for constructing recombinant DNA molecules and analyzing genetic structures. Additionally, NruI is employed in restriction mapping to determine the location of specific sequences within a DNA molecule, aiding in genome studies and diagnostics. Its specificity and efficiency make it a key tool in biotechnology and genetic research.