Endonuclease StuI is a restriction enzyme that specifically recognizes the palindromic DNA sequence AGGCCT and cleaves it bluntly between the central A residues. It is widely used in molecular biology for DNA manipulation and analysis. It is particularly valuable in genetic engineering for cutting DNA at specific recognition sites, enabling the creation of recombinant DNA molecules. This enzyme is essential in cloning experiments, where it helps in the precise insertion of DNA fragments into vectors. Additionally, Endonuclease StuI is utilized in restriction fragment length polymorphism (RFLP) analysis to study genetic variations and identify mutations. Its role in DNA fingerprinting and genome mapping further highlights its importance in research and diagnostic applications. The enzyme's specificity and efficiency make it a key tool in advancing studies in genetics and biotechnology.