Endonuclease TaqI is widely used in molecular biology for DNA manipulation and analysis. It is particularly valuable in restriction fragment length polymorphism (RFLP) studies, where it helps in identifying genetic variations by cutting DNA at specific recognition sites. This enzyme is also employed in cloning experiments to digest DNA into smaller fragments, facilitating the insertion of genes into vectors. Additionally, TaqI is utilized in polymerase chain reaction (PCR) applications for preparing DNA templates and analyzing PCR products. Its thermostable nature makes it suitable for use in high-temperature environments, enhancing its efficiency in various genetic engineering and diagnostic procedures.