Used extensively in fluorescence labeling and imaging, this chemical is a vital tool in biological research. It helps in tracking and visualizing cellular processes due to its bright green fluorescence. Commonly applied in flow cytometry, it aids in cell sorting and analysis. It is also utilized in fluorescence microscopy to study live cells and tissues. The compound is effective in nucleic acid staining, enabling the detection of DNA and RNA in various assays. Additionally, it serves as a marker in enzyme activity studies, particularly in assays involving proteases and peptidases. Its high sensitivity and stability make it a preferred choice for diagnostic applications and high-throughput screening.