5(6)-ROX is widely used as a fluorescent dye in various biological and chemical applications. It is particularly valuable in labeling and detection processes due to its high fluorescence intensity and stability. In molecular biology, it is commonly employed in fluorescence in situ hybridization (FISH) to visualize and map genetic material in cells. Additionally, it serves as a tracer in flow cytometry and fluorescence microscopy, enabling researchers to study cellular structures and processes with high precision. The dye is also utilized in nucleic acid sequencing and real-time PCR (polymerase chain reaction) as a reporter molecule, providing accurate and sensitive detection of target sequences. Its compatibility with a range of excitation and emission wavelengths makes it a versatile tool in both research and diagnostic applications.