Used extensively as a fluorescent dye in biochemical and cellular research, it helps in labeling and tracking biomolecules due to its high fluorescence intensity. It is particularly valuable in fluorescence microscopy and flow cytometry for visualizing cellular processes. Additionally, it serves as a substrate in enzyme activity assays, where its fluorescence increases upon enzymatic cleavage, enabling real-time monitoring of enzyme kinetics. Its application also extends to nucleic acid detection, where it is used to label probes for in situ hybridization, aiding in the study of gene expression and localization.