Agarose Gel Loading Buffer-Sucrose is primarily used in molecular biology for the analysis of nucleic acids through gel electrophoresis. It serves as a medium to prepare DNA or RNA samples for loading into agarose gels. The sucrose component increases the density of the sample, ensuring it sinks evenly into the wells of the gel. This buffer also contains dyes, such as bromophenol blue or xylene cyanol, which migrate through the gel alongside the nucleic acids, providing visual tracking of the electrophoresis progress. By using this buffer, researchers can accurately separate and analyze DNA or RNA fragments based on size, which is essential for applications like PCR product verification, restriction enzyme digestion analysis, and other genetic studies.