Sepharose CL-6B is widely used in biochemistry and molecular biology for the purification and separation of biomolecules. It is particularly effective in gel filtration chromatography, where it helps separate proteins, nucleic acids, and other macromolecules based on their size. This is especially useful for isolating large molecules or complexes, such as antibodies, enzymes, and polysaccharides. Its cross-linked structure provides excellent mechanical stability, making it suitable for use in both low-pressure and high-performance liquid chromatography systems. Additionally, Sepharose CL-6B is often employed in affinity chromatography when coupled with specific ligands to purify target molecules with high specificity. Its versatility and reliability make it a key tool in research, diagnostics, and industrial bioprocessing.