Sephadex G150 is widely used in biochemical laboratories for the separation and purification of biomolecules based on their size. It is particularly effective in gel filtration chromatography, where it helps isolate proteins, peptides, and other macromolecules from complex mixtures. The application of Sephadex G150 is crucial in protein purification processes, enabling researchers to obtain high-purity samples for downstream analysis or use. It is also employed in desalting procedures, where it separates proteins from small molecules like salts or detergents. Additionally, Sephadex G150 is utilized in molecular weight estimation, as its porous structure allows for the differentiation of molecules based on their size. Its stability and compatibility with aqueous solutions make it a reliable tool in various biotechnological and pharmaceutical applications.