Galactosylcerebrosides (non-hydroxy) are primarily utilized in the field of biochemistry and medical research. These compounds play a significant role in studying cell membrane structure and function, particularly in the nervous system. They are often used as biomarkers for diagnosing certain neurological disorders, such as Krabbe disease, where the accumulation of these lipids indicates a metabolic abnormality. Additionally, they are employed in research to understand the mechanisms of lipid metabolism and the role of glycosphingolipids in cellular signaling pathways. Their application extends to the development of therapeutic strategies targeting lipid-related diseases, making them crucial in both diagnostic and therapeutic contexts.