Dihydroxyacetone phosphate dilithium salt is widely used in biochemical research and enzymatic studies. It serves as a key intermediate in glycolysis and gluconeogenesis pathways, making it essential for understanding cellular metabolism. Researchers utilize it to study enzyme kinetics, particularly those of aldolase and triosephosphate isomerase, which are crucial in carbohydrate metabolism. Additionally, it is employed in the synthesis of complex carbohydrates and in metabolic pathway analysis. Its stability and solubility make it a preferred choice for in vitro experiments and as a substrate in diagnostic assays for metabolic disorders.