Rhodanese from bovine liver is primarily utilized in biochemical research and enzymatic studies due to its ability to catalyze the transfer of sulfur atoms from thiosulfate to cyanide, converting toxic cyanide into less harmful thiocyanate. This property makes it valuable in studying detoxification pathways, particularly in understanding how organisms mitigate cyanide poisoning. It is also employed in laboratory assays to measure enzyme activity and to investigate sulfur metabolism. Additionally, rhodanese has applications in environmental science for developing methods to detoxify cyanide-contaminated sites. Its role in sulfur transfer reactions further contributes to research in protein biochemistry and enzyme kinetics.