(R)-Plevitrexed is primarily used in the field of oncology as an anticancer agent. It functions as a thymidylate synthase inhibitor, which is crucial for DNA synthesis and repair in cells. By inhibiting this enzyme, it effectively halts the proliferation of cancer cells, making it particularly useful in the treatment of various solid tumors, including colorectal and pancreatic cancers. Its application is often explored in clinical trials to evaluate its efficacy and safety in combination with other chemotherapeutic agents or as a standalone treatment. The compound’s targeted mechanism of action helps in reducing the side effects commonly associated with traditional chemotherapy, offering a more refined approach to cancer therapy.